Plant Radiation Hybrid Mapping
We are exploring the use of plant interspecific radiation hybrids for the development of chromosome-based physical maps. In our system, we start with plants where a chromosome has been introduced in an alien background (the addition lines). Because a chromosome in an alien background has been physically separated from chromosomes of its original genome, analysis of this single chromosome represents a dramatic reduction of the complexity of materials to be manipulated. Further reduction in complexity may be achieved by irradiating an addition line and isolating lines that possess different pieces of the chromosome of interest. These sub-chromosome fragment stocks (known as radiation hybrids) can then be used to study a specific region of the chromosome in question or to re-construct the structure of the chromosome of interest. In collaboration with Dr. M. Isabel Vales, we are investigating the inheritance and stability of maize-chromosome 9 rearrangements from a panel of radiation hybrids. In collaboration with Dr. Shahryar Kianian (North Dakota State University), we are also developing a radiation hybrid panel to localize scs, a gene that affects nucleo-cytoplasmic interactions in wheat.
Publications
Kalavacharla, V., K.G Hossain, Y. Gu, O. Riera-Lizarazu, M.I. Vales, S. Bhamidimarri, J.L. Gonzalez-Hernandez, S.S. Maan, and S.F. Kianian. 2006. High-resolution radiation hybrid map of wheat chromosome 1D. Genetics 173:1089-1099.
Vales, M.I., O. Riera-Lizarazu, H.W. Rines, and R.L. Phillips. 2004. Transmission of maize chromosome 9 rearrangements in oat-maize radiation hybrids. Genome 47: 1202–1210.
Hossain, K.G., O. Riera-Lizarazu, V. Kalavacharla, M.I. Vales, J.L. Rust, S.S.Maan, and S.F. Kianian. 2004. Molecular cytogenetic characterization of an alloplasmic durum wheat line with a portion of chromosome 1D of Triticum aestivum carrying the scsae gene. Genome 47: 206-214.
Hossain, K.G., O. Riera-Lizarazu, V. Kalavacharla, M.I. Vales, S.S.Maan, S.F. Kianian. 2004. Radiation hybrid mapping of the species cytoplasm specific (scsae) gene in wheat. Genetics 168: 415-423.
Riera-Lizarazu, O., M. I. Vales, E. V. Ananiev, H. W. Rines, and R. L. Phillips. 2000. Production and characterization of maize chromosome 9 radiation hybrids derived from an oat-maize addition line. Genetics 156:327-339.