TitleCycloheximide prevents the de novo polypeptide synthesis required to recover from acetylene inhibition in Nitrosopumilus maritimus.
Publication TypeJournal Article
Year of Publication2014
AuthorsVajrala, N, Bottomley, PJ, Stahl, DA, Arp, DJ, Sayavedra-Soto, LA
JournalFEMS Microbiol Ecol
Volume88
Issue3
Pagination495-502
Date Published2014 Jun
ISSN1574-6941
KeywordsAcetylene, Ammonia, Archaea, Cycloheximide, Nitrification, Nitrosomonas europaea, Oxidation-Reduction, Protein Synthesis Inhibitors
Abstract

Developing methods to differentiate the relative contributions of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) to ammonia (NH3) oxidation has been challenging due to the lack of compounds that selectively inhibit AOA. In this study, we investigated the effects of specific bacteria- and eukaryote-selective protein synthesis inhibitors on the recovery of acetylene (C2H2)-inactivated NH3 oxidation in the marine AOA Nitrosopumilus maritimus and compared the results with recovery of the AOB Nitrosomonas europaea. C2 H2 irreversibly inhibited N. maritimus NH3 oxidation in a similar manner to what was observed previously with N. europaea. However, cycloheximide (CHX), a widely used eukaryotic protein synthesis inhibitor, but not bacteria-specific protein synthesis inhibitors (kanamycin and gentamycin), inhibited the recovery of NH3-oxidizing activity in N. maritimus. CHX prevented the incorporation of (14)CO2 -labeling into cellular proteins, providing further evidence that CHX acts as a protein synthesis inhibitor in N. maritimus. If the effect of CHX on protein synthesis can be confirmed among other isolates of AOA, the combination of C2H2 inactivation followed by recovery of NH3 oxidation either in the presence of bacteria-selective protein synthesis inhibitors or CHX might be used to estimate the relative contributions of AOB and AOA to NH3 oxidation in natural environments.

DOI10.1111/1574-6941.12316
Alternate JournalFEMS Microbiol. Ecol.
PubMed ID24606542