|Overexpression of acetyl-CoA synthetase in Saccharomyces cerevisiae increases acetic acid tolerance.
|Year of Publication
|Ding, J, Holzwarth, G, Penner, MH, Patton-Vogt, J, Bakalinsky, AT
|FEMS Microbiol Lett
|Acetate-CoA Ligase, Acetic Acid, Acetyl Coenzyme A, Fermentation, Gene Expression Regulation, Fungal, Genes, Fungal, Plasmids, Real-Time Polymerase Chain Reaction, Saccharomyces cerevisiae
Acetic acid-mediated inhibition of the fermentation of lignocellulose-derived sugars impedes development of plant biomass as a source of renewable ethanol. In order to overcome this inhibition, the capacity of Saccharomyces cerevisiae to synthesize acetyl-CoA from acetic acid was increased by overexpressing ACS2 encoding acetyl-coenzyme A synthetase. Overexpression of ACS2 resulted in higher resistance to acetic acid as measured by an increased growth rate and shorter lag phase relative to a wild-type control strain, suggesting that Acs2-mediated consumption of acetic acid during fermentation contributes to acetic acid detoxification.
|FEMS Microbiol. Lett.
|PubMed Central ID
|R15 GM104876 / GM / NIGMS NIH HHS / United States
R15GM104876 / GM / NIGMS NIH HHS / United States