TitleOverexpression of acetyl-CoA synthetase in Saccharomyces cerevisiae increases acetic acid tolerance.
Publication TypeJournal Article
Year of Publication2015
AuthorsDing, J, Holzwarth, G, Penner, MH, Patton-Vogt, J, Bakalinsky, AT
JournalFEMS Microbiol Lett
Date Published2015 Jan
KeywordsAcetate-CoA Ligase, Acetic Acid, Acetyl Coenzyme A, Fermentation, Gene Expression Regulation, Fungal, Genes, Fungal, Plasmids, Real-Time Polymerase Chain Reaction, Saccharomyces cerevisiae

Acetic acid-mediated inhibition of the fermentation of lignocellulose-derived sugars impedes development of plant biomass as a source of renewable ethanol. In order to overcome this inhibition, the capacity of Saccharomyces cerevisiae to synthesize acetyl-CoA from acetic acid was increased by overexpressing ACS2 encoding acetyl-coenzyme A synthetase. Overexpression of ACS2 resulted in higher resistance to acetic acid as measured by an increased growth rate and shorter lag phase relative to a wild-type control strain, suggesting that Acs2-mediated consumption of acetic acid during fermentation contributes to acetic acid detoxification.

Alternate JournalFEMS Microbiol. Lett.
PubMed ID25673654
PubMed Central IDPMC4809976
Grant ListR15 GM104876 / GM / NIGMS NIH HHS / United States
R15GM104876 / GM / NIGMS NIH HHS / United States