A retrospective study of the prevalence and classification of intestinal neoplasia in zebrafish (Danio rerio).

TitleA retrospective study of the prevalence and classification of intestinal neoplasia in zebrafish (Danio rerio).
Publication TypeJournal Article
Year of Publication2013
AuthorsPaquette, CE, Kent, ML, Buchner, C, Tanguay, RL, Guillemin, K, Mason, TJ, Peterson, TS
Date Published2013 Jun
KeywordsAdenocarcinoma, Age Factors, Animal Feed, Animals, Asymptomatic Infections, Carcinoma, Small Cell, Diet, Female, Fish Diseases, Intestinal Neoplasms, Male, Prevalence, Retrospective Studies, Sex Factors, Zebrafish

For over a decade, spontaneous intestinal neoplasia has been observed in zebrafish (Danio rerio) submitted to the ZIRC (Zebrafish International Resource Center) diagnostic service. In addition, zebrafish displayed preneoplastic intestinal changes including hyperplasia, dysplasia, and enteritis. A total of 195 zebrafish, representing 2% of the total fish submitted to the service, were diagnosed with these lesions. Neoplastic changes were classified either as adenocarcinoma or small cell carcinoma, with a few exceptions (carcinoma not otherwise specified, tubular adenoma, and tubulovillous adenoma). Tumor prevalence appeared similarly distributed between sexes and generally occurred in zebrafish greater than 1 year of age, although neoplastic changes were observed in fish 6 months of age. Eleven lines displayed these preneoplastic and neoplastic changes, including wild-types and mutants. Affected zebrafish originated from 18 facilities, but the majority of fish were from a single zebrafish research facility (hereafter referred to as the primary facility) that has submitted numerous samples to the ZIRC diagnostic service. Zebrafish from the primary facility submitted as normal sentinel fish demonstrate that these lesions are most often subclinical. Fish fed the diet from the primary facility and held at another location did not develop intestinal lesions, indicating that diet is not the etiologic agent.

Alternate JournalZebrafish
PubMed ID23544991
PubMed Central IDPMC3673592
Grant ListT32 RR023917 / RR / NCRR NIH HHS / United States
R24 RR017386 / RR / NCRR NIH HHS / United States
P01HD22486 / HD / NICHD NIH HHS / United States
P40RR012546 / RR / NCRR NIH HHS / United States
R24 OD010998 / OD / NIH HHS / United States